Step 1 : Source Plate: Sample aspirated and pocket in tip fills to capacity (50 nL, or 250 nL).
Step 2 : Source Plate: Sample dispensed back to cherry pick plate and pocket remains full.
Step 3 : Wash Plate: The portion of the tip below the pocket is washed to eliminate carryover.
Step 4 : Destination Plate: Sample/liquid in destination (assay) plate is aspirated to pocket level in the tip, and mixed.
Step 5 : Destination Plate: Mixed sample is dispensed back to the destination (assay) plate.